lepr protein  (Human Protein Atlas)

Journal: Biomaterials Research

Article Title: Oxidative Stress and Hormone-Regulated Dermal Papilla Cell-Targeted Nanomodulators: Reverse Cellular Senescence for Androgenetic Alopecia Therapy

doi: 10.34133/bmr.0333

Figure Lengend Snippet: Schematic illustration of the fabrication process (A) and therapeutic AGA mechanism (B) of DPC-targeted nanocarriers (L-LP-Fi/CeNPs) that synergistically inhibit DPC senescence. DPC, dermal papilla cell; PEG, polyethylene glycol; CeNP, cerium oxide nanoparticle; Fi, finasteride; L-LP, leptin-functionalized, co-loaded liposome; DP, dermal papilla; DHT, dihydrotestosterone; ROS, reactive oxygen species; HFSC, hair follicle stem cell; TGF-β, transforming growth factor β; AGA, androgenetic alopecia; LEPR, leptin receptor; T, testosterone; SOD, superoxide dismutase; CAT, catalase.

Article Snippet: Intracellular LEPR was labeled with a rabbit anti-LEPR protein monoclonal antibody (1:100, HUABIO), followed by an Alexa Fluor 561 goat anti-rabbit secondary antibody incubation.

Techniques:

Journal: Biomaterials Research

Article Title: Oxidative Stress and Hormone-Regulated Dermal Papilla Cell-Targeted Nanomodulators: Reverse Cellular Senescence for Androgenetic Alopecia Therapy

doi: 10.34133/bmr.0333

Figure Lengend Snippet: LEPR expression in DPCs and validation of binding to leptin. (A) LEPR expression in HDPCs, HaCaT cells, MDPCs, and mEK cells. Scale bar = 50 μm. (B) Quantitative analysis of LEPR expression in cells ( n = 3). (C) Time scale analysis of the HF cycle in C57BL/6 mice at 11 weeks after birth. (D) LEPR expression in different HF phases of mice. Scale bar = 25 μm. (E and F) Western blotting and grayscale analysis of LEPR expression (HDPC and HaCaT) ( n = 3). (G) The mechanism by which leptin targets LEPR-expressing DPCs. (H) CLSM imaging following co-incubation with FITC-labeled leptin (green) and Alexa Fluor 647-labeled LEPR (red). Scale bar = 20 μm. (I) Grayscale colocalization analysis of the images shown in (H), (a) PCC (HDPC) = 0.5298 and (b) PCC (MDPC) = 0.5263. All results are presented as means ± SDs (* P < 0.05 and *** P < 0.001). DAPI, 4′,6-diamidino-2-phenylindole; HF, hair follicle; HDPC, human dermal papilla cell; MDPC, mouse dermal papilla cell; mEK, mouse epidermal keratinocyte; FITC, fluorescein isothiocyanate; PCC, Pearson correlation coefficient.

Article Snippet: Intracellular LEPR was labeled with a rabbit anti-LEPR protein monoclonal antibody (1:100, HUABIO), followed by an Alexa Fluor 561 goat anti-rabbit secondary antibody incubation.

Techniques: Expressing, Biomarker Discovery, Binding Assay, Western Blot, Imaging, Incubation, Labeling

Journal: Biomaterials Research

Article Title: Oxidative Stress and Hormone-Regulated Dermal Papilla Cell-Targeted Nanomodulators: Reverse Cellular Senescence for Androgenetic Alopecia Therapy

doi: 10.34133/bmr.0333

Figure Lengend Snippet: Transdermal retention and cellular uptake of L-LPs. (A) CLSM images of pig skin after the application of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (Dil)-labeled LPs or L-LPs for 12 and 24 h. Scale bar = 200 μm. (B) In vitro skin retention of liposomes and free drug ( n = 3). (C) FCM analysis and CLSM images of DPCs exposed to LPs or L-LPs for 2 or 6 h. Scale bar = 50 μm ( n = 3). (D) Colocalization images of LEPR and L-LPs. Scale bar = 100 μm (* P < 0.05 and *** P < 0.001). CLSM, confocal laser scanning microscope; FCM, flow cytometry.

Article Snippet: Intracellular LEPR was labeled with a rabbit anti-LEPR protein monoclonal antibody (1:100, HUABIO), followed by an Alexa Fluor 561 goat anti-rabbit secondary antibody incubation.

Techniques: Labeling, In Vitro, Liposomes, Laser-Scanning Microscopy, Flow Cytometry

Journal: Biomaterials Research

Article Title: Oxidative Stress and Hormone-Regulated Dermal Papilla Cell-Targeted Nanomodulators: Reverse Cellular Senescence for Androgenetic Alopecia Therapy

doi: 10.34133/bmr.0333

Figure Lengend Snippet: LEPR expression in DPCs and validation of binding to leptin. (A) LEPR expression in HDPCs, HaCaT cells, MDPCs, and mEK cells. Scale bar = 50 μm. (B) Quantitative analysis of LEPR expression in cells ( n = 3). (C) Time scale analysis of the HF cycle in C57BL/6 mice at 11 weeks after birth. (D) LEPR expression in different HF phases of mice. Scale bar = 25 μm. (E and F) Western blotting and grayscale analysis of LEPR expression (HDPC and HaCaT) ( n = 3). (G) The mechanism by which leptin targets LEPR-expressing DPCs. (H) CLSM imaging following co-incubation with FITC-labeled leptin (green) and Alexa Fluor 647-labeled LEPR (red). Scale bar = 20 μm. (I) Grayscale colocalization analysis of the images shown in (H), (a) PCC (HDPC) = 0.5298 and (b) PCC (MDPC) = 0.5263. All results are presented as means ± SDs (* P < 0.05 and *** P < 0.001). DAPI, 4′,6-diamidino-2-phenylindole; HF, hair follicle; HDPC, human dermal papilla cell; MDPC, mouse dermal papilla cell; mEK, mouse epidermal keratinocyte; FITC, fluorescein isothiocyanate; PCC, Pearson correlation coefficient.

Article Snippet: Following a 24-h incubation, the mouse skin was subjected to cryosectioning and subsequently labeled with a rabbit monoclonal antibody specific to LEPR proteins (dilution 1:100; HUABIO), followed by incubation with an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody.

Techniques: Expressing, Biomarker Discovery, Binding Assay, Western Blot, Imaging, Incubation, Labeling